The quantitative effects of cadherins are crucial, but qualitative interactions—that is, the type and timing of cadherin expression—also can be important. The timing of particular developmental events can depend on cadherin expression. For instance, N-cadherin appears in the mesenchymal cells of the developing chick leg just before these cells condense and form nodules of cartilage (which are the precursors of the limb skeleton). N-cadherin is not seen prior to condensation, nor is it seen afterward. If the limbs are injected just prior to condensation with antibodies that block N-cadherin, the mesenchyme cells fail to condense and cartilage fails to form (Oberlender and Tuan 1994). It therefore appears that the signal to begin cartilage formation in the chick limb is the appearance of N-cadherin.
The type of cadherin can matter as well. Duguay and colleagues (2003) showed, for instance, that R-cadherin and B-cadherin do not bind well to each other. When two populations of cells expressing either R-cadherin or B-cadherin at equal levels are mixed together, they sort out into two opposing mounds of cells with a distinct border between them (Figure 1A). The formation of boundaries is a critical physical achievement necessary for many morphogenetic events. For instance, in the developing ectoderm, the expression of N-cadherin is important in separating the precursors of the neural cells from the precursors of the epidermal cells (Figure 1B). Initially, all early embryonic cells contain E-cadherin, but those cells destined to become the neural tube lose E-cadherin and gain N-cadherin. If epidermal cells are experimentally made to express N-cadherin or if N-cadherin synthesis is blocked in prospective neural cells, the border between the skin and the nervous system fails to form properly (Figure 1A; Kintner et al. 1992). Thus, through the differential expression of two different cadherin types, different tissues can become separated by the formation of a border at the cell membrane occupying the weaker heterophilic interaction (Fagotto 2014).
Another example of boundary formation in the embryo occurs within the mesoderm to separate the axial (notochordal) mesoderm from the paraxial (somitic) mesoderm. The primary mechanism for forming this boundary rests in the reduction of C-cadherins in the opposing membranes of the border cells (Fagotto et al. 2013). Fagotto and colleagues examined this mechanism in live Xenopus laevis embryos and found that actin-myosin contractile cables line up parallel to the border interface and are required for both C-cadherin reduction and boundary formation (Figure 2).