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Return to Principles of Development 6e Student Resources
Chapter 3 Self-test questions
Vertebrate development I: life cycles and experimental techniques
In which portion of the frog's life cycle would a frog appear most similar to a mammal?
The notochord is a ___ structure in vertebrate embryos that lies under the ___, and is flanked by ___.
mesodermal, neural tube, somites
endodermal, mesoderm, the gut
ectodermal, neural tube, mesoderm
mesodermal, ectoderm, endoderm
The darkly pigmented end of a
egg is called
the animal region
the vegetal region
Which of the following choices puts the early developmental stages of the frog in their correct order?
blastula, fertilized egg, gastrula, neurula
cleavage, gastrulation, neurulation, organogenesis
neurulation, gastrulation, cleavage, organogenesis
tadpole, embryo, neurula, gastrula
During gastrulation in
, the blastocoel
becomes the gut
is filled with endodermal cells and disappears
does not change, but develops into what is called the archenteron
is displaced, and its original location becomes an endoderm-lined cavity, the archenteron, which is a precursor to the gut
The process in which the three germ layers form is called:
Cleavage in the zebrafish gives rise to the
The primitive streak of mouse and chick embryos is equivalent to which amphibian structure?
In chick development, the cell movement through the primitive streak during gastrulation is called:
At the blastocyst stage, the mammalian embryo is composed of
a blastocoel, yolk, and animal cap cells
an epiblast overlying a hypoblast
ectodermal, mesodermal, and endodermal cells
trophectoderm and inner cell mass
In mammalian development, the embryo will form from which population of cells?
the inner cell mass
the extraembryonic membranes
All the genes expressed in a particular tissue or at a particular stage of development can be identified using
fusion of adjacent cells using an electrical pulse
injection of a gene into the nucleus of a cell
the introduction of a fluorescent lineage maker into cells
use of brief pulses of electricity to cause cells to take up DNA
The least favorable model organism for traditional genetic analysis is
What property of the mammalian embryo permits the production of chimeric mice?
The inner cell mass is highly regulative, so that extra cells derived from the inner cell mass of a different embryo are incorporated without causing defects.
If half of the cells of the inner cell mass are removed and replaced with cells from half of a different embryo's inner cell mass, the embryo will heal and develop normally.
The embryo can develop outside the womb, making surgical manipulations possible.
Blastomeres separated at the two-cell stage will regulate and each forms a normal embryo.
Which technique is commonly used for gene knock-out in mice?
microinjection of GFP
RNA interference (RNAi)
The advantage of using the Cre/
system for targeted mutagenesis is
a gene can be deleted in a particular tissue or at a desired time in development
system allows germline transgenesis in amphibians and chickens
system is more specific than homologous recombination
the deletion of a gene by the Cre/
system is reversible
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